Oligonucleotide-functionalized gold nanoparticles (DNA-Au NPs) find ubiquitous use in various biomolecular detection schemes due to their unique chemical and physical properties. This study investigates the effects of nanoparticle size and concentration of salt in solution on the DNA loading of the Au NP surface. The DNA loading studies are performed by functionalization of the Au NPs with fluorophore-labeled DNA. The Au NPs are further utilized for the detection of prostate specific antigen (PSA) using a highly sensitive bio-barcode assay. The amplification of the signal in the biobarcode assay is proportional to the number of barcode strands per particle. Therefore, the determination of the barcode oligonucleotide loading on the Au NPs at different conditions is a crucial step in the optimization of the bio-barcode assay

Last modified

• 07/18/2018

Creator

• Jae-Seung Lee
• Angel S. Butron
• Brian Stepp
• SonBinh Nguyen
• Savka I. Stoeva
• Chad A. Mirkin

DOI

• https://doi.org/10.21985/N22428

Keyword

• Volume 4

Date created

• 2007

Resource type

• Article

Rights statement

• In Copyright