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Filtering by: 2019-01-01 Creator Adam D. Silverman

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Source Data for "Point-of-use detection of environmental fluoride via a cell-free riboswitch-based biosensor."

Description:

Advances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that...

Keyword:

Diagnostic, Fluoride, Riboswitch, Cell-Free, and Synthetic Biology

Subject:

Synthetic Biology and Diagnostics

Creator:

Nancy Kelley-Loughnane, Matthew S. Verosloff, Michael C. Jewett, Walter Thavarajah, Julius B. Lucks, and Adam D. Silverman

Contributor:

This work was supported by the Air Force Research Laboratory Center of Excellence for Advanced Bioprogrammable Nanomaterials (C-ABN) Grant FA8650-15-2-5518 (to M.C.J. and J.B.L), the David and Lucile Packard Foundation (to M.C.J.), an NSF CAREER Award (1452441 to J.B.L.), and the Camille Dreyfus Teacher-Scholar Program (to M.C.J. and J.B.L.). A.D.S. was supported in part by the National Institutes of Health Training Grant (T32GM008449) through Northwestern University’s Biotechnology Training Program.

Owner:

Julius Beau Lucks

Language:

English

Date Uploaded:

06/18/2019

Date Modified:

01/08/2020

Date Created:

2019-06-04

Resource Type:

Dataset

Alternate Identifier:

https://doi.org/10.21985/N2RJ64
Design and optimization of a cell-free atrazine biosensor

Description:

Recent advances in cell-free synthetic biology have spurred the development of in vitro molecular diagnostics that serve as effective alternatives to whole-cell biosensors. However, cell-free sensors for detecting manmade organic water contaminants such as pesticides are sparse, partially because few characterized natural biological sensors can directly detect such pollutants. Here,...

Keyword:

biosensor, TX-TL, atrazine, cell-free, cyanuric acid, metabolism, synthetic biology, and transcription factor

Creator:

Umut Akova, Khalid K. Alam, Michael C. Jewett, Julius B. Lucks, and Adam D. Silverman

Owner:

Adam Silverman

Date Uploaded:

09/23/2019

Date Modified:

02/19/2020

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Source Data for "Point-of-use detection of environmental fluoride via a cell-free riboswitch-based biosensor."

Design and optimization of a cell-free atrazine biosensor

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